ExCellerate NK Media

ExCellerate™

Animal-Component Free NK Media

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500×500 NK cell

Accelerate Your Development of NK Cell Therapies

Natural Killer (NK) cells play an essential role in adaptive and innate immune responses, and due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells are critical to facilitate NK cell therapy.

ExCellerate Human NK Cell Expansion Media is animal-component free (RUO) available in 1L bottles.  Development of the biosimilar GMP Media in bags and bottles is in production.

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ExCellerate NK Media Competitor Comparisons

Exceptional Expansion; Feeder & Feeder-Free

  • Supports feeder- and serum-free expansion of NK cells
  • Phenol red-free formulation
  • Animal component-free media to support patient safety
  • Supports NK cell expansion from PBMCs or purified CD3+-depleted PBMCs
  • Protocol to support G-Rex feeder-cell culture process

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ExCellerate media support expansion and growth of gene-edited NK cells with TcBuster

Across a sampling of donors, it can be seen that non-viral TcBuster gene-edited NK cells show comparative fold expansion and growth against non-edited.  Cumulative expansion outperforms non-edited cells in most cases.  CAR expression also yields high quality and quantity when pairing TcBuster with ExCellerate NK Media.

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Why Choose ExCellerate NK Media?

ExCellerate NK Media offers a robust and versatile solution for NK cell cultures, designed to seamlessly transition from research to clinical applications. This media is formulated without any animal components and is both phenol-red free and serum-free, aligning with the latest standards in cell culture practices. Its equivalent formulation to the GMP media (CCM037-GMP), set to launch in FY24 in both bottles and bags, ensures a smooth scale-up process.
Animal-Component Free Formulation

Exceptional Expansion. High Viability, High Killing Capacity.

Multiple Protocols. Optimized for G-Rex.
Gene-Edited NK Cell Cultures

Serum-Free Culture with Feeder-Based Platform

ExCellerate NK Media not only facilitates exceptional expansion of NK cells but also maintains their high viability and killing capacity. This media supports a serum-free process, which is crucial for highly effective NK cell cultures. Its compatibility extends to various cell types, including iPSC-derived NK cells, PBMCs, and purified NK cells, and it can be used in different cell culture systems, including G-Rex culture vessels. Suited for both engineered and non-engineered platforms, it also works well with non-viral gene editing systems like TcBuster™. Additionally, ExCellerate NK Media is optimized for use with Bio-Techne tools and reagents, making it a comprehensive solution for NK cell culture needs.
>10,000 NK expansion​

>1000 NK expansion​. Higher expansion rate by lowering IL-2 concentration to 250 IU/mL​
Maintained CD16 and NKG2D expression

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500×500 NK Media

Versatile NK Cell Expansion Media

ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from Bio-Techne R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody.

The media supports both feeder-based and feeder-free expansion methods.

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How to order

Fill out the form below or contact us directly to place an order or consult with a ScaleReady Optimization Specialist.
Phone: 651-628-9259
Email: info@scaleready.com
Fax: 651-628-9507

Additional Resources

Determining Optimal Seeding Density for Human T Cell Expansion in G-Rex

This scientific poster investigates the kinetics of human T cell expansion in small scale G-Rex6M bioreactors using ScaleReady reagents. Data show the affect of seeding density on fold expansion, total cell yield, and phenotypic phenotypic composition of expanded T cells, including total and memory CD4+ and CD8+ cells.
View Poster

G-Rex Closed-system Bioreactors

In this brochure you will learn how G-Rex bioreactors solve the limitations of flask and bag-based cell therapy manufacturing. In this brochure:
  • G-Rex Bioreactor mechanism of action
  • Benefits for cell therapy manufacturing
  • GatheRex Cell Harvest Pump
  • User testimonials
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ScaleReady Portfolio Overview

We’ve anticipated where cell therapy manufacturing needs to be and we’re the only solution for cost-effective and practical cell therapy scale-up. In this brochure:
  • Portfolio introduction
  • G-Rex Bioreactors and raw materials
  • Cell processing instruments
Read Brochure

See how GMP Cytokines fit into the ScaleReady™ workflow.

Explore the Workflow