ExCellerate NK Media

ExCellerate™

Animal-Component Free NK Media

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Accelerate Your Development of NK Cell Therapies

Natural Killer (NK) cells play an essential role in adaptive and innate immune responses, and due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells are critical to facilitate NK cell therapy.

ExCellerate Human NK Cell Expansion Media is animal-component free (RUO) available in 1L bottles.  Development of the biosimilar GMP Media in bags and bottles is in production.

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Exceptional Expansion; Feeder & Feeder-Free

  • Supports feeder- and serum-free expansion of NK cells
  • Phenol red-free formulation
  • Animal component-free media to support patient safety
  • Supports NK cell expansion from PBMCs or purified CD3+-depleted PBMCs
  • Protocol to support G-Rex feeder-cell culture process

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ExCellerate media support expansion and growth of gene-edited NK cells with TcBuster

Across a sampling of donors, it can be seen that non-viral TcBuster gene-edited NK cells show comparative fold expansion and growth against non-edited.  Cumulative expansion outperforms non-edited cells in most cases.  CAR expression also yields high quality and quantity when pairing TcBuster with ExCellerate NK Media.

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Why Choose ExCellerate NK Media?

ExCellerate NK Media offers a robust and versatile solution for NK cell cultures, designed to seamlessly transition from research to clinical applications. This media is formulated without any animal components and is both phenol-red free and serum-free, aligning with the latest standards in cell culture practices. Its equivalent formulation to the GMP media (CCM037-GMP), set to launch in FY24 in both bottles and bags, ensures a smooth scale-up process.
Animal-Component Free Formulation

Exceptional Expansion. High Viability, High Killing Capacity.

Multiple Protocols. Optimized for G-Rex.
Gene-Edited NK Cell Cultures

Serum-Free Culture with Feeder-Based Platform

ExCellerate NK Media not only facilitates exceptional expansion of NK cells but also maintains their high viability and killing capacity. This media supports a serum-free process, which is crucial for highly effective NK cell cultures. Its compatibility extends to various cell types, including iPSC-derived NK cells, PBMCs, and purified NK cells, and it can be used in different cell culture systems, including G-Rex culture vessels. Suited for both engineered and non-engineered platforms, it also works well with non-viral gene editing systems like TcBuster™. Additionally, ExCellerate NK Media is optimized for use with Bio-Techne tools and reagents, making it a comprehensive solution for NK cell culture needs.
>10,000 NK expansion

>1000 NK expansion​. Higher expansion rate by lowering IL-2 concentration to 250 IU/mL
Maintained CD16 and NKG2D expression

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Versatile NK Cell Expansion Media

ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from Bio-Techne R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody.

The media supports both feeder-based and feeder-free expansion methods.

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How to order

Fill out the form below or contact us directly to place an order or consult with a ScaleReady Optimization Specialist.
Phone: 651-628-9259
Email: info@scaleready.com
Fax: 651-628-9507

NK Media Sample Request form

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Additional Resources

Determining Optimal Seeding Density for Human T Cell Expansion in G-Rex

This scientific poster investigates the kinetics of human T cell expansion in small scale G-Rex6M bioreactors using ScaleReady reagents. Data show the affect of seeding density on fold expansion, total cell yield, and phenotypic phenotypic composition of expanded T cells, including total and memory CD4+ and CD8+ cells.

G-Rex Closed-system Bioreactors

In this brochure you will learn how G-Rex bioreactors solve the limitations of flask and bag-based cell therapy manufacturing. In this brochure:
  • G-Rex Bioreactor mechanism of action
  • Benefits for cell therapy manufacturing
  • GatheRex Cell Harvest Pump
  • User testimonials

ScaleReady Portfolio Overview

We’ve anticipated where cell therapy manufacturing needs to be and we’re the only solution for cost-effective and practical cell therapy scale-up. In this brochure:
  • Portfolio introduction
  • G-Rex Bioreactors and raw materials
  • Cell processing instruments

See how GMP Cytokines fit into the ScaleReady™ workflow.